Safety in Industrial Microbiology and Biotechnology by C. H. Collins
By C. H. Collins
This can be a assessment of the secure dealing with of microbes and organic fabric in undefined. Biotechnology is a brand new and growing to be undefined, and even supposing a few governments have arrange regulatory our bodies and either the OECD and the EC have issued files, so much legitimate instructions are directed at laboratory instead of at commercial purposes. while there are fears in regards to the topic one of the basic public in regards to the attainable health and wellbeing dangers of biotechnological operations and an uncertainty between employees in at quite a few degrees approximately secure and hazardous practices
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The probability of such an occurrence was studied experimentally. Mice are very susceptible to the oncogenic polyoma virus, which also can cause tumours in baby hamsters. Israel et al. (1979a) cloned the complete genome of polyoma virus (Py) in E. coli K12 and injected 5 x 109 of such bacteria into mice. 6. The logarithm of the number of Py-DNA equivalents, necessary for infection, is plotted on the abscissa. The bottom line (no. 1) shows that about 200 virus particles or Py equivalents are necessary for an infection (MID = mean infective dose or ID 50 ).
And a Working Party of the European Federation of Biotechnology (1985) Safe biotechnology I. General considerations. Applied Microbiology and Biotechnology, 21, 1-6 Nederlandse Vereniging voor Microbiologie (1984) Aanbevelingen voor Veilig Microbiologisch Werk, 2nd edn, Bilthoven NIH (1974) National Institutes of Health Biohazards Safety Guide, US Goverment Printing Office, Washington DC NIH (1978) National Institutes of Health Laboratory Safety Monograph. Supplement to the NIH Guidelines for Recombinant DNA Research, US Government Printing Office, Washington DC Hazard groups and containment categories in microbiology and biotechnology 33 NIH (1986) Guidelines for Research Involving Recombinant DNA Molecules.
E. coli K12 did not become pathogenic after the introduction of the genes for an adherence factor and for enterotoxin (Guinée, 1977) or for adherence and penetration (Isberg and Falkow, 1985). To obtain an oral vaccine against dysentery, eight known virulence factors of Shigella were transferred into E. coli K12. None of 100 volunteers taking 1010 of these viable hybrids orally became ill. , 1977). 5 Genes for growth in the body (P) occur dispersed on the chromosome of a pathogen. Accidental cloning of five such factors in the same plasmid has an unrealistic probability (P=10~ 15 ) These arguments are also valid for Saccharomyces cerevisiae, Bacillus subtilis and other harmless saprophytes that do not grow in the human body.