Integrin and Cell Adhesion Molecules: Methods and Protocols by Alexandre Chigaev, Larry A. Sklar (auth.), Motomu Shimaoka

By Alexandre Chigaev, Larry A. Sklar (auth.), Motomu Shimaoka (eds.)

Integrins play pivotal roles not just throughout a variety of physiological strategies together with tissue morphogenesis, immune responses, wound therapeutic, and rules of mobilephone progress and differentiation, but in addition in different pathological phenomena comparable to autoimmunity, thrombosis, and melanoma metastasis/progression. as a result, investigations on integrins frequently call for multi-disciplinary ways, making researchers lengthy for a convenient number of finished and sensible protocols that aspect experimental equipment for learning integrin and comparable mobile adhesion molecule performance. Integrin and telephone Adhesion Molecules: equipment and Protocols aims to supply readers not just with uncomplicated protocols in learning integrin features, but additionally with summaries on these cutting-edge applied sciences which were applied for figuring out integrin performance on the mobile, molecular, structural, and organismal degrees. Divided into six handy sections, this designated quantity covers easy protocols for the examine of integrin and comparable telephone adhesion molecule performance in vitro, illustrates structural biology methods for learning integrins and similar mobile adhesion molecules, specializes in rising imaging applied sciences for investigating telephone migration, offers innovations to explain signaling via mobilephone adhesion molecules, comprises experimental innovations to enquire integrin features at organismal degrees in a physiological context, and showcases the main promising tools and applied sciences for the advance of novel therapeutics and diagnostics. Written within the winning Methods in Molecular Biology™ sequence structure, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, simply reproducible protocols, and notes on troubleshooting and heading off recognized pitfalls.

Both specialists and non-experts within the medical neighborhood who desire to examine mobile adhesion molecules and diagnostics will locate Integrin and mobilephone Adhesion Molecules: tools and Protocols authoritative, simply obtainable, and significantly informative.

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Immunol. 178, 3903–3911. Alon, R. and Feigelson, S. W. (2009) Chemokine signaling to lymphocyte integrins under shear flow. Microcirculation. 16, 3–16. Alon, R. and Ley, K. (2008) Cells on the run: shear-regulated integrin activation in leukocyte rolling and arrest on endothelial cells. Curr. Opin. Cell Biol. 20, 525–532. Ley, K. (2009) Cell adhesion under flow. Microcirculation. , Bleijs, D. , Lobb, R. , and Alon, R. (2000) Subsecond induction of alpha4 integrin clustering by immobilized chemokines stimulates leukocyte tethering and rolling on endothelial vascular cell adhesion molecule 1 under flow conditions.

Wagner, C. , and Sklar, L. A. (2005) Dissociation of I domain and global. conformational changes in LFA-1: refinement of small molecule-I domain structure-activity relationships. Biochemistry 44, 4322–4331. , Zwartz, G. , Edwards, B. , Prossnitz, E. , and Sklar, L. A. (2004) Conformational regulation of alpha 4 beta 1-integrin affinity by reducing agents. “Inside-out” signaling is independent of and additive to reduction-regulated integrin activation. J. Biol. Chem. 279, 32435–32443. , Lopez, G.

Weitz-Schmidt and S.  3. Effect of inhibitors on PBMC binding to ICAM-1 in the V-well adhesion assay. The activity of the small molecule LFA-1 inhibitor LFA878 (a) and the anti-ICAM-1 mAb (b) was tested at indicated concentrations in the V-well assay, as described under Subheading 3. Each point or bar represents the mean value ±SD of triplicates. Blank, no ICAM-1; control: plus ICAM-1, no inhibitor. Both inhibitors blocked cell adhesion to immobilized ligand in a concentration-dependent manner.

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