Basic Methods in Antibody Production and Characterization by Gary C. Howard (Editor), Delia R. Bethell (Editor)

By Gary C. Howard (Editor), Delia R. Bethell (Editor)

Written for researchers and execs within the fields of biomedical study, immunology, biochemistry, molecular biology, pathology, and biotechnology, easy tools in Antibody creation and Characterization makes use of a cookbook method of featuring the tools for the construction, characterization, and use of antibodies. Antibodies defined comprise polyclonal and monoclonal and people made by means of the phage exhibit. uncomplicated tools in Antibody creation and Characterization specializes in particular facets of antibodies and takes readers on a step by step approach from antigen instruction to immunizations, adjuvants, screening, purification, garage, and applications.Introductory fabric accompanies every one bankruptcy, giving readers a greater realizing of the tools and purposes. plentiful references arm researchers with many functions touching on the actual know-how.

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45, 1, 1995. 10. Maloy, W. , Coligan, J. , Production of antipeptide antisera, in Current Protocols in Immunology, Vol. 1, Coligan, J. , Kruisbeek, A. , Margulies, D. , Shevach, E. , John Wiley & Sons, New York, 1994, chap. 4. 11. van de Wijgert, J. , Verheul, A. , Check, I. , and Hunter, R. , Immunogenicity of Streptococcus pneumoniae type 14 capsular polysaccharide: influence of carriers and adjuvants on isotype distribution, Infect. , 59, 2750, 1991. 12. Glenn, G. , Richards, R. , Matyas, G.

3. Calculate the amount of antigen required and the volume desired per injection. Allow 10 to 100 µg of antigen per mouse, in a volume of 200 µl per mouse for IM or SC injections. For IP injections, allow 200 to 500 µl volume per mouse. Detergents will weaken the emulsion and should be avoided. 4. Place the capped cryovial into an adjustable clamp. Align it directly below the stirring rod allowing space to remove the vial’s cap. 5. Warm CFA to 37°C prior to use and vortex vigorously for 1 to 2 min to resuspend the M.

6,7,8 TiterMax® is a water-in-oil emulsion developed specifically for the production of antibodies in research animals. TiterMax has three primary ingredients: (1) a block copolymer, CRL 89-41; (2) squalene, a metabolizable oil; and (3) a microparticulate silica. The nonionic block copolymer is a synthetic immunomodifier which acts in part by adhesion of protein antigens to oil, thereby enhancing delivery 9445_frame_C04 Page 21 Monday, July 31, 2000 7:44 AM Adjuvants 21 of high concentrations of antigen-to-antigen presenting cells.

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