Applied Virology Research: New Diagnostic Procedures by Christine Kurstak, A. Hossain, Edouard Kurstak (auth.),

By Christine Kurstak, A. Hossain, Edouard Kurstak (auth.), Edouard Kurstak, R. G. Marusyk, F. A. Murphy, M. H. V. Van Regenmortel (eds.)

Volume three is dedicated to the newest diagnostic expertise for virus illnesses, relatively molecular methodologies.

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Steps C and D are repeated to achieve exponential amplification of the ligated products AB and A'B'. 38 D. Y. R. ~ ~RT 2 3 CycJe I ot TAS .................... .. .... '"' - -J' • l' -3· -J' ANA ANA AHA AHA s·- - - - - - - - -:r ANA s·- ...... - .. - ... ,. ~" s·- ... - - ... - - s·- - ... - - - - S'- ... "" - ) - .... • ~ > Rl DNA ! < 6 --:-- ~: :; ! , . ~ . B TranacrllltiOI'Il ~. ~ 4 s·- - - - - - - - -:s· ANA • .. • - - .... - .. •s I 6' • ... • l ' • - .. S' - - &' • .... A ... . . . .

VII. , 1989). , 1989). , 1989). , 1989) has been unable to verify the original results (unpublished data) and will require further investigation. , 1989). , 1989). PBMCS from these men were analyzed for HIV-1 DNA by PCR using primers from the env and gag regions of the viral genome. Using a Markov statistical model, it was estimated that the median time from infection with HIV-1 to seroconversion was within 3 months and that 95% of all persons who became infected would seroconvert within 6 months.

The extreme sensitivity of the PCR process has the potential to amplify a nucleic acid sequence in a sample that is negative by all other criteria. Finally, multiple reagent controls should be included with each amplification. This is because the presence of a small number of molecules of PCR product in the reagents may lead to sporadic positive results. The reagent controls should contain all of the necessary components for the PCR process minus the template DNA. CONCLUSION As the AIDS epidemic continues to grow and spread, there will be an increasing need for sensitive and quantitative assays for HIV.

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