A Laboratory Guide to the Mammalian Embryo by David K. Gardner, Michelle Lane, Andrew J. Watson

By David K. Gardner, Michelle Lane, Andrew J. Watson

This e-book pulls jointly the total variety of telephone tradition, biochemical, microscopic, and genetic options to review the early mammalian embryo. before, there hasn't ever been this kind of accomplished compendium, notwithstanding there were extra targeted books of protocol, resembling Manipulating the Mouse Embryo, from chilly Spring Harbor. This publication is meant to entice all constituencies, from simple experimental technology to medical and animal technology functions.

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Leese, H. , and Gosden, R. G. (1993). Biol. , 48, 798. Murray, A. , Gosden, R. , and Spears, N. (1998). J. Reprod. , 113, 27. Ryle, M. (1969). J. Reprod. , 20, 307. Greenwald, G. , and Moor, R. M. (1989). J. Reprod. , 87, 561. , and Winston, R. M. (1999). Hum. , 14, 2519. Wright, C. , Winston, R. , and Hardy, K. (1999). Hum. , 14, 1555. , and Tuuri, T. (2001). Mol. Hum. , 6, 694. Hovatta, O. (2001). Mol. , 169, 95. , and Gosden, R. G. (1998). Fertil. , 69, 1. Picton, H. , and Gosden, R. G. (2000).

0275 g sodium pyruvate. 5. 06 g penicillin. 6. Add 4 g BSA. 7. 8 g of NaOH. 05. 8. 2-mm filter. Maturation TCM-199/MEM stock solution 1. 2. 3. 4. 5. 6. Measure out 1 l of sterile 18Wohm water. Add 1 bottle of TCM-199/MEM to the 1 l of water; rinse the bottle three times. 420 g NaHCO3. 0275 g sodium pyruvate. 06 g penicillin. 2-mm filter. 2-mm low protein binding filter. 11. 5 g 2,2,2-tribromoethanol. Store in dark at room temperature. Working solution: 10 ml saline, 120 ml stock solution. May need to warm to 37°C to dissolve.

02 ml/g body weight. Vasectomy 1. Anesthetize the male and place him ventral side up on the microscope stage. 02 ml/g body weight. 2. Spray the area between the anus and penis with 70% alcohol. 3. Make an incision about 2–3 mm long ventrally along the scrotal sac below the penis through the body wall and peritoneum. 4. Locate the bursa sac containing the testes and gently tear a hole at the bottom of the sac near the epididymus. 5. Locate the epididymus and pull through the hole in the bursa. 6.

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